Objective: To investigate hepatoprotective activity of aqueous extract of Salvadora persica against paracetamol intoxication in rats and its correlation with antioxidant activity. Methods: In vitro lipid peroxidation was determined by Ohkawa H et al., 1979 and hydroxyl radical scavenging activity was determined by deoxyribose degradation method in comparison to standard ascorbic acid. Albino Wister rats of either sex weighing between 150-250 g were divided into five groups. Group 1 received normal saline. Groups 2 to 5 received 2 g/kg paracetamol for 3 days and then groups 3 and 4 received 200 mg/kg and 400 mg/kg of aqueous extract of Salvadora persica whereas group 5 received silymarin 100 mg/kg from 4th day to 10 day. On 0th day (one day before the dosing) and 11th day blood was collected by retro orbital puncture. Serum was separated and biochemical parameters measured. Results: IC50 value of the aqueous extract of Salvadora persica was found to be329.67μg and 351.57 μg and 191.63 μg and 184.96 μg for ascorbic acid respectively for lipid peroxidation and hydroxyl radical activity. Administration of aqueous extract of Salvadora persica (200 and 400 mg/kg) markedly reduced the biochemical parameters. Average percentage change in these parameters was significantly reduced on 11th day. Conclusion:Aqueous extract of Salvadora persica possessed significant hepatoprotective activity, in accordance with its in vitro antioxidant activity. The in vitro antioxidant activity further provides support to the hepatoprotective activity and provides insight in understanding the probable mechanism of action of the Salvadora persica as hepatoprotective.